Metabolomics and Lipidomics Core

Thermo Orbitrap Exploris™ 480 Mass Spectrometer

Thermo Orbitrap Exploris™ 480 Mass Spectrometer coupled to either a Vanquish Duo (for electro-spray ionization (ESI)) or a Vanquish Neo liquid chromatography system (for micro and nano-flow ESI).

Overview

The metabolome and lipidome in a given biological system provide a molecular snapshot of the rapid and complex metabolic perturbations associated with life.  Their analysis may be crucial for the understanding of the mechanisms of health and disease. The use of mass spectrometry for metabolomics and lipidomics research has increased exponentially over the past two decades, providing a highly sensitive analytical tool for quantitative analysis of molecules in complex biological matrices. The Metabolomics and Lipidomics core facility provides expertise and state-of-the-art mass spectrometry instrumentation and analytical methods for such analyses.

Services

  • Metabolomics: Both “untargeted” and library based polar metabolomics covering relative quantification of central carbon metabolism, amino acids, and other major metabolites. Standardized assay with limited flexibility in provided output.
  • Lipidomics: Non-polar metabolite analysis providing relative quantification of major structural, intermediate, and signaling lipids. Standardized assay with limited flexibility in provided output.
  • Method Development: Analytical method development and validation for targeted analysis requiring high and known degrees of analytical parameters including sensitivity and specificity.  Allows less standard methods to be developed and tested.
  • Consultations
  • Training

Equipment

Thermo Orbitrap Exploris™ 480 Mass Spectrometer

Coupled to either a Vanquish Duo (for electro-spray ionization (ESI)) or a Vanquish Neo liquid chromatography system (for micro and nano-flow ESI).

Thermo Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer

Coupled to a Vanquish Duo for ESI/APCI, and a Waters XEVO TQ Absolute coupled to a Waters I-class for ESI/Atmospheric Pressure Chemical Ionization (APCI) or Waters micro-binary solvent manager for desorption electrospray ionization (DESI).

Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer

Coupled to a Waters I-class for ESI/APCI or Waters micro-binary solvent manager for DESI.

Specialized small equipment

necessary for sample preparation and handling, including BSL-2 space

Contact Information

Katz users can visit the Katz Portal to learn more about these services and how to access them. For external users: please contact the Manager.

Director/Manager
Director: Tomasz Skorski, MD PhD
Manager: Andrea Andress Huacachino, PhD
Manager: Mariola M. Marcinkiewicz, MS

Metabolomics and Lipidomics Core Location:
Facility: 480 MERB (Medical Education and Research Building)
Office: 408 MERB (Medical Education and Research Building)
Hours of Operation: Monday - Friday 9:30 am-5:30 pm.