Bioimaging Core

Zeiss Lightsheet 7

The Zeiss Lightsheet 7 is a fast, low-phototoxicity light-sheet fluorescence microscope designed for high-resolution 3D imaging.

Overview

Advanced cellular imaging techniques allow researchers to visualize cells, ex vivo tissues, and interior structures within living animals with incredible detail and in real-time. This capacity opens up new pathways for discovery across basic and translational research, allowing better understanding of human diseases. The mission of the Bioimaging Core is to make state-of-the-art imaging technologies available to researchers.

Services

  • Microscopy & Imaging Services
    The Core provides comprehensive support for all imaging platforms, including light-sheet, confocal, TIRF, widefield, and long-term live-cell imaging systems. Users receive guidance on fluorophore selection, optical configurations, and imaging strategies tailored to their biological questions. Assisted imaging is available for complex imaging, and the Core offers expertise in data acquisition, quantitative 3D/4D analysis, stitching, deconvolution, segmentation, and generation of publication-ready figures and movies.

  • Training and Certification for Independent Use
    The Core offers structured training and certification programs to ensure that researchers can operate microscopes independently and safely. New users receive hands-on instruction covering sample preparation, instrument setup, imaging optimization, and data management. Certification is granted once users demonstrate proficiency in instrument operation and adherence to Core policies and best practices. This process enables qualified users to reserve microscopes for unassisted use, ensuring high-quality data and consistent experimental performance across all platforms.

  • Tissue Clearing and 3D Immunolabeling Services
    Large-volume tissue clearing and immunolabeling are performed by the Manager using the Lifecanvas SmartBatch+ system, which automates all steps from SHIELD preservation to refractive-index matching. The system processes up to twelve organs or thick tissues simultaneously with precise temperature and fluidic control, resulting in uniformly cleared and labeled samples ready for downstream imaging. These workflows support whole-brain and spinal cord clearing, DRG and peripheral nerve studies, tumor microenvironment mapping, vascular and lymphatic imaging, organoid analysis, and other applications requiring intact-tissue 3D molecular profiling.

Equipment

Zeiss Lightsheet 7

A fast, low-phototoxicity light-sheet microscope for high-resolution 3D imaging of live or fixed, cleared samples. This instrument features dual-sided illumination, refractive-index matching, major clearing-protocol compatibility, 405/488/561/638 nm lasers, and 5×/10× illumination with 5×/20× detection lenses, enabling uniform volumetric imaging of organs, organoids, embryos, tumors, and vasculature.

Zeiss Celldiscoverer 7 with LSM 900 & Airyscan 2

The Celldiscoverer 7 is an automated live-cell imaging system combining widefield, confocal, and Airyscan 2 super-resolution with a motorized incubator for long-term, low-phototoxicity imaging. It features LEDs (385–625 nm), confocal lasers (405/488/561/640 nm), 5× and 20× air objectives, a 50× water lens, and a 0.5×/1×/2× magnification changer, supporting high-content and 3D imaging of organoids, trafficking, and cytoskeletal dynamics.

Leica SP8 Confocal Microscope

The Leica SP8 is a sensitive, spectrally flexible confocal system with HyD detectors and a tunable white-light laser. It provides 405/488/552/638 nm lasers, multiple air, oil, water, and glycerol objectives, fast resonant scanning, and advanced modes such as FRAP, FRET, and photoactivation for high-resolution live-cell and molecular imaging.

Zeiss Laser TIRF 3 Microscope

The Zeiss Laser TIRF 3 provides ultra-thin evanescent-field illumination for imaging events within ~100 nm of the plasma membrane. It includes 488 and 532/561 nm lasers, 100× oil TIRF lenses, and 20×/40×/63× oil objectives for epi and phase imaging. With GFP/Cy3/Cy5 filter sets and full environmental control, it enables high-contrast live-cell imaging of receptor signaling, endocytosis, exocytosis, cytoskeletal dynamics, and fast vesicle events.

Leica DM6000B Stereology Microscope

The Leica DM6000B is an automated upright stereology microscope with motorized stage control and high-quality optics (2.5×, 5×, 10×, 20×, 40×, and 63×). This instrument supports brightfield and fluorescence imaging and integrates with MBF Bioscience software for unbiased tissue quantification, commonly used for neuronal counts, glial analysis, tumor burden, and developmental/toxicologic histology.

Image-Analysis Workstations

The Imaging Core provides high-performance workstations equipped with Imaris 9.6.1 for 3D/4D visualization and tracking, Arivis Pro 4.2 for large volumetric and light-sheet datasets, ZEN Blue 3.8 for acquisition-compatible analysis, and ZEN Desk 3.11 for offline processing, documentation, and annotation. These tools support advanced segmentation, volumetric quantification, stitching, deconvolution, and production of publication-quality figures and movies.

Contact Information

Katz users can visit the Katz Portal to learn more about these services and how to access them. For external users: please contact the Manager.

Director/Manager
Director: Young-Jin Son, Ph.D.
Manager: Hyukmin Kim, Ph.D.

Bioimaging Core Locations

Medical Education and Research Building (MERB)
672 (Confocal, laser microdissection microscope) 
681C (Lightsheet, live-cell confocal/superresolution microscope) 
681(SmartBatch+ Clearing system) 
674 (Stereology microscope) 
681, 681C (Workstations with Imaris, Zen Desk, Arivis)

Hours of Operation: Monday - Friday 9am-5pm