The Chromium instrument automates the cell partitioning and barcoding steps which are critical in the single cell workflow. Utilizing advanced GEM-X microfluidics, it generates from hundreds to over a million single cell partitions in just minutes, with each partition containing an identifying barcode for downstream analysis. The Chromium X is the most advanced of the Chromium instrument series, offering access to a full portfolio of assays that capture multiple molecular readouts, including gene expression, epigenetic analysis (e.g. chromatin accessibility), proteins, immune clonotype, and analysis of CRISPR perturbation screens. It is used upstream of the next-generation sequencing (NGS) system.

The Tapestri Single-cell Multiomics Sequencing Platform uses a droplet microfluidic workflow that partitions individual cells, analyzing both DNA and proteins. The Tapestri uses pre-configured or custom gene panels and an automated workflow including isolation of DNA and oligo-conjugate antibodies, followed by targeted multiplex PCR. The instrument enables the simultaneous detection of genetic variants, including single nucleotide variants (SNVs), insertions/deletions (indels), copy number variants (CNVs), and loss of heterozygosity (LOH), together with expression of cell surface proteins (for instance, for immunological profiling) at single-cell resolution. This is also used upstream of the next-generation sequencing (NGS) system.

The NextSeq 2000 NGS sequencing platform offers broad options for data output and sequencing read length, giving access to a wide range of applications.

The facility contains additional equipment, including an Agilent BioAnalyzer 2100, ThermoFisher Qubit 2.0 and KAPA Illumina Library Quantification, which are used for quality control during sample preparation.