Instrumentation
Analyzers
LSR II
- 4 lasers (488 nm, 407 nm, 633 nm, 355 nm), 13 fluorescent channels
- Digital acquisition using DiVa software, automatic offline compensation
- Uses fiber optics and Octagon/Trigon detector arrays to increase sensitivity
- Detector configuration
Sorters
Aria IIµ
- 3 lasers (488 nm, 407 nm, 633 nm), 8 fluorescent channels
- Digital acquisition using DiVa software, automatic offline compensation
- Uses fiber optics and Octagon/Trigon detector arrays to increase sensitivity
- Cuvette-based flow cell, more sensitive than jet-in-air cytometer
- Up to 4-way sorting, single cell sorting into 96 well plates
- Nozzle size and sorting pressure can be changed for different types of cells. Available nozzles: 70 µm, 85 µm, 100 µm. Larger nozzle size results in lower sheath pressure, and the cells are less stressed. However, the sort is slower and collected cells are in greater volume of PBS.
- Detector configuration
Influx with HEPA-filtered enclosure
- 4 lasers (488 nm, 407 nm, 633 nm, 561 nm), 14 fluorescent channels
- Analog to digital acquisition
- 561 nm laser excites PE and PE tandem dyes better than 488 nm laser. It also excites red fluorescent proteins, such as mCherry, DsRed.
- Forward Scatter detector is Photo Multiplier Tube (PMT) instead of Diode, more sensitive for particles < 1 µm
- Jet-in-air cytometer
- Up to 4-way sorting, single cell sorting into 96 well plates
- Nozzles: 70 µm, 100 µm, 140 µm, 200 µm.
- BSL-2 sorting including live human cells
- Detector configuration
Analysis Workstation
- Mac Pro computer with Flowjo and CellQuest Pro software
- CD/DVD writer